Actual algae, or a case of mistaken identity? If we are of the belief that black algae is actually an alga as the name suggests, then the answer is that we do not know this microscopic pain in the ass very well at all. This is a huge contributing factor in the depth of the struggle pool operators face in remedying this scourge once established. Repeatedly treating something that is not algae as if it were an algae will continuously yield poor results. The truth of the matter, as we will explain below, is that the name “Black Algae” is merely a misnomer.
The cells in black algae do not contain a formed nucleus, because of this, they can not be in the protista (algae) kingdom. So, if black algae are not algae, what are they? Black algae, often-dubbed blue-green algae (to confuse matters further) actually belong to the classification prokaryote. Prokaryotic cells are simple and without organelles that are typically found in eukaryotic cells. Bacteria, however, are prokaryotic cells. That’s right, those slimy little plaster pimples are actually a type of bacteria. The scientific name for black algae is cyanobacteria, sometimes referred to as cyanophyta. To our defense, cyanobacteria were once thought to be algae.
WIth over 150 genera of cyanobacteria and a scarcity in scientific studies available, I would need to conduct my own research in order to determine exactly what and which type we are finding established in swimming pools. The analysis would also serve to support my statement above identifying “black algae” as cyanophyta. To locate samples, I reached out to my CPO Certification Class Alumni to see if any had taken on a new pool that possibly had colinization. Success! On March 30 of 2018, I gathered several 1/6 ounce screwcap vials and a 30 ml ampoule of Lugol’s Solution – Yes, I just happen to have these things. I carefully removed samples of the photosynthetic prokaryotes by gently scraping for placement into the vials. I then added the Lugol’s solution for preservation. In a third vial (Shown at page top) the collected bacteria was contained in water from the pool vs. the Lugol’s in order to have one live colony among those carefully preserved.
With specimens in hand, I stopped in to see my friend Professor Edward Phlips Ph. D in his Phycology Research Lab at the University of Florida’s Center for Aquatic and Invasive Plants. Dr. Phlips (BA in Biology, University of California; MS, Biological Oceanography, University of Miami; PhD, Marine Biology, University of Miami) serves as professor of algal physiology and ecology at the university. Doctor Phlips is one of the Country’s foremost experts on algae. Unfortunately, due to a planktonic bloom in the Indian River Lagoon, I would not have priority status at the microscope.
Dr. Phlips was able to confirm my suppositions. The dime-sized samples of black algae I had collected were identified as thick matted layers comprised of not one, but several genus of cyanobacteria (blue/green algae) in an intertwined colony. Oscillatoria, Microcoleous, and Nostoc were the most prevalent. Here we have classified only genus, each of which have several hundred species. Of the three genus mentioned, Dr. Phlips found the discovery of Nostoc within the thimble-sized clusters removed from the swimming pool wall to be the most interesting, stating that “Nostoc is a nitrogen-fixing cyanobacterium “. Though we did not test specifically for other harmful pathogens amongst the cyanobacterium, the UF Phycologist agreed that it was possible other bateria and/or protozoa may take harbor within the mass. Testing for the presence of cyanotoxins was not performed, however certain species of Oscillatoria and Nostoc are known to be toxigenic, naturally releasing toxins. Others have been known only to release cyanotoxins upon rupture (lysis) of the cell membrane itself.
According to The University of California, Berkeley, cyanobacteria have the distinction of being the oldest known fossils at more than 3.5 billion years old. Aside from being a living fossil, an added defense the bacteria possess is that they have an accessory cell wall of polymers, which create a protective outer membrane. This outer layer allows them a greater level of resistance against chemicals we would typically use as algaecides and for disinfection in swimming pool maintenance. Colonization only adds additional layers of protection to the bacteria’s level of resistance to chlorine.
Regarding the analysis of the samples we collected:The sample of “black algae” was examined using light microscopy (by Susan Badylak, Senior Biological Scientist). The sample consisted of a tightly bound mat of filamentous algae imbedded in mucilage. The mat was teased apart for more detailed examination of species composition. The dominant species in the mat were filamentous cyanobacteria, also known as blue-green algae. Two genera were observed, the nitrogen-fixing form Nostoc sp. and non-nitrogen fixing forms Microcoleus (as well as Oscillatoria mentioned earlier). Nitrogen fixation is a process which allows certain groups of cyanobacteria to thrive in environments where the availability of nitrogen, an essential growth nutrient, is limited. Both genera are known to contain species which form dense mats. Some species of Nostoc are known to produce toxins, but the potential for toxin production by the species in the sample would require more specific and detailed analysis of additional samples…” – Edward J. Phlips, Professor, Fisheries and Aquatic Sciences Program. SFRC, University of Florida
This discovery of Nostoc sp. is an important discovery for the pool industry because this helps to explain the challenges in inactivating this tenaciousness trespasser. We chlorinate to destroy organic contaminants; oxidize ammonia and nitrogen compounds; and to santize and disinfect. Nitrogen is an important component to the growth of all living things and is absolutely essential in the ability to perform photosynthesis. The genus we have discovered (Nostoc sp.) simply produces its own food when none is available, explaining why phosphate removers and reduction of nitrates does not work well for “Black Algae” treatment. This gives this cyanophyta the ability to thrive in a properly maintained swimming pool and aggravate the pool operator on a heightened level.
One of the unique characteristics about cyanobacteria that set it apart from other bacteria is the fact that this bacterium does have some plant-like/protist-like characteristics. Specifically, cyanobacteria contain chloroplasts and perform photosynthesis, just as plants/protists do. Meaning that, these microbes utilize sunshine, water and C02 (carbon dioxide) to produce both carbohydrates and oxygen. – Scientific American credits cyanobacteria as “The Origin of Oxygen in Earth’s Atmosphere”. This use of chlorophyll may serve as the prokaryote’s Achilles’ heel for us as pool professionals.
This is where we recognize the benefits of heavy metal. No, James Hetfield of Metallica is not going to treat “Black Algae” problems as they arise. This is about the other type of heavy metals, specifically copper and silver. Quaternary algaecides (quaternary ammonium compounds) are truly weapons of algae destruction and have had some reported success in the elimination of cyanobacteria. Withal, the doses necessary for toxicity were well in excess of the recommended application rates. Though the recomended dose can prevent the microorganisms from becoming established and, if added with colonization present, can stifle the growth. This is discussed in Dr. Phlip’s 1992 research doc (Phlips, Edward & Hansen, Phyllis & Velardi, Tom): Effect of the herbicide diquat on the growth of microalgae and cyanobacteria. Bulletin of environmental contamination and toxicology. 49. 750-6. 10.1007/BF00200790.
Others have reported success by sprinkling calcium hypochlorite directly over the colonies (plaster pools only), or simply by rubbing a trichlor tablet upon it (plaster pools only). There are actually even “Trichlor Tablet Holders” available that enable the pool operator to attach a 3″ tablet to a telepole specifically for this purpose. These methods will achieve the desired results as long as the cyanobacteria have taken residence only upon the pool floor, or if established in only a few tiny clusters communities. Still, this chalking and/or sprinkling method will only treat what one can see; not treating the pool as a whole. Remember, both algae and bacteria are microscopic and you do not actually see them until there are thousands upon thousands in a colony.
Copper is essential to photosynthetic organisms, however in large amounts it can prove fatal to the bacteria. Copper is absolutely necessary to perform photosynthesis, in excess the metal will actually inhibit the process. Without the ability to synthesize food from CO2 (carbon dioxide) and energy from the sun, the bacteria, like any photosynthetic organism, will not survive. Copper will also cause lysis to occur (rupturing of the cell membrane). Therefore, the impact of a copper algaecide in the treatment of cyanobacteria is two-fold, damaging the bacteria from both inside and out.
The oligodynamic effect also comes into play. This refers to the biocidal effect of metals. Certain heavy metals have been found to kill bacteria and viruses upon contact, copper having a slightly higher kill rate than silver. Silver ions are believed to alter proteins within bacteria which results in complete inactivation. In systems that utilize both copper and silver, it is the belief that the copper penetrates the cell wall (lysis) providing the silver with an entry point expediting the annihilation of the nefarious nostoc with a one-two punch. What exactly happens within a cell in the presence of heavy metals (The oligodynamic effect) is still theorized, with thoughts ranging from DNA, RNA, enzyme and cellular protein destruction; to hydroxyl radical formation. All of which are beyond the scope of this discussion.
Ozone, permanganate, chlorine, chlorine dioxide, and UV have also proven successful in the treatment of cyanobacteria in varius concentrations. Both Ozone and UV are more effective when used in conjunction with Hydrogen Peroxide, though Hydrogen Peroxide alone is ineffective. Success in utilizing these methods has been, in many cases, achieved in testing involving single cells vs. colonized cyanobacteria. All of the methods above are dependent upon pH, temperature, and contact time.
“Rudy, Your field samples are very interesting – and rare. There is almost nothing published on the varieties of algae in pools… If you focus on black “algae” you will be the first person in nearly 40 years to document it. That would be really, really cool.” – Dr. Roy D. Vore, Ph.D., Microbial physiologist, Technology Manager, BioLab inc.
“Fascinating!!!” – Dr. Tom Lachocki. Ph.D, Chief Executive Officer, National Swimming Pool Foundation
👾 Results of Copper Sulfate field study in the treatment of cyanobacteria: Click Here
A shout out to my references:
Special Thanks to my G-ville peeps:
Professor Ed Phlips, Ph.D, UF Phycology Dept
Susan Badylak, Senior Biological Scientist, Department of Fisheries and Aquatic Sciences, University of Florida
Kristin Wilson, Scientist – for keeping my technobabble user-friendly